產(chǎn)品名稱：Anti-Transferrin receptor抗體,轉(zhuǎn)鐵蛋白受體抗體
產(chǎn)品編號：BYK-0988R
英文名稱：Anti-Transferrin receptor
中文名稱：轉(zhuǎn)鐵蛋白受體抗體
英文別名：CD 71; CD71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; Transferrin receptor protein 1, serum form; TRFR.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細胞術(shù)等實驗（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Transferrin receptor抗體,轉(zhuǎn)鐵蛋白受體抗體
英文介紹：Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro.
此產(chǎn)品本公司有標記的一抗出售，相關(guān)標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-Transferrin receptor抗體,轉(zhuǎn)鐵蛋白受體抗體封閉(Blocking):
轉(zhuǎn)膜完畢后，立即把蛋白膜放置到預先準備好的Western洗滌液中，漂洗1-2分鐘，以洗去膜上的轉(zhuǎn)膜液。從轉(zhuǎn)膜完畢后所有的步驟，一定要注意膜的保濕，避免膜的干燥，否則極易產(chǎn)生較高的背景。
用微型臺式真空泵吸盡洗滌液，加入Western封閉液，在搖床上緩慢搖動,室溫封閉60分鐘。對于一些背景較高的抗體，可以在4℃ 封閉過夜。
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