鏈珯瀹㈡埗鏈嶅嫏(w霉)铏�(h脿o)
鐠�(hu谩n)淇濆湪绶氬畼寰�
鐠�(hu谩n)淇濆湪绶氭墜姗�(j墨)鐗�
鐠�(hu谩n)淇濆湪绶欰PP
鐠�(hu谩n)淇濆湪绶氬皬绋嬪簭
涓婃捣鍗″姫鐢熺墿绉戞妧鏈夐檺鍏徃浣滆��
鐗涘綀鎬ц泲鐧介叾锛圗lastase锛夐叾鑱�(li谩n)鍏嶇柅鍒嗘瀽 ELISA瑭﹀姂鐩�
| 璩囨枡椤炲瀷 | 鏂囦欢 | 璩囨枡澶у皬 | 0 |
| 涓嬭級娆℃暩(sh霉) | 41 | 璩囨枡鍦栫墖 | 銆愰粸(di菐n)鎿婃煡鐪嬨�� |
| 涓� 鍌� 浜� | 涓婃捣鍗″姫鐢熺墿绉戞妧鏈夐檺鍏徃 | 闇�瑕佺⿳鍒� | 0 |
| 闂�(gu膩n) 閸� 瑭� | 鐗涘綀鎬ц泲鐧介叾锛圗lastase锛夐叾鑱�(li谩n)鍏嶇柅鍒嗘瀽,涓婃捣鍗″姫鐢熺墿,ELISA瑭﹀姂鐩� ,48T/96T | ||
- 銆愯硣鏂欑啊浠嬨��
瑭﹀姂鐩掍娇鐢ㄨ鏄庢浉
鏈│鍔戝儏渚涚爺绌朵娇鐢� 鐩殑锛氭湰瑭﹀姂鐩掔敤浜庢脯瀹氱墰琛�娓咃紝琛�婕垮強鐩搁棞(gu膩n)娑查珨妯ｆ湰涓�褰堟�ц泲鐧介叾锛�Elastase锛�鐨勫惈閲忋��
瀵�(sh铆)椹�(y脿n)鍘熺悊锛�
鏈│鍔戠洅鎳�(y墨ng)鐢ㄩ洐鎶楅珨澶惧績娉曟脯瀹氭(bi膩o)鏈腑鐗涘綀鎬ц泲鐧介叾锛�Elastase锛夋按骞炽�傜敤绱斿寲鐨勭墰褰堟�ц泲鐧介叾锛�Elastase锛夋姉楂斿寘琚井瀛旀澘锛屽埗鎴愬浐鐩告姉楂旓紝寰�鍖呰鍠姉鐨勫井瀛斾腑渚濇鍔犲叆褰堟�ц泲鐧介叾锛�Elastase锛夛紝鍐嶈垏HRP妯�(bi膩o)瑷樼殑褰堟�ц泲鐧介叾锛�Elastase锛�鎶楅珨绲�(ji茅)鍚堬紝褰㈡垚鎶楅珨-鎶楀師-閰舵(bi膩o)鎶楅珨寰�(f霉)鍚堢墿锛岀稉(j墨ng)閬�*娲楁粚鍚庡姞搴曠墿TMB椤壊銆�TMB鍦�HRP閰剁殑鍌寲涓嬭綁(zhu菐n)鍖栨垚钘�(l谩n)鑹诧紝骞跺湪閰哥殑浣滅敤涓嬭綁(zhu菐n)鍖栨垚zui绲傜殑榛冭壊銆傞鑹茬殑娣辨泛鍜屾ǎ鍝佷腑鐨�褰堟�ц泲鐧介叾锛�Elastase锛�鍛堟鐩搁棞(gu膩n)銆傜敤閰舵(bi膩o)鍎�鍦�450nm娉㈤暦涓嬫脯瀹氬惛鍏夊害锛�OD鍊硷級锛岄�氶亷妯�(bi膩o)婧�(zh菙n)鏇茬窔瑷�(j矛)绠楁ǎ鍝佷腑鐗涘綀鎬ц泲鐧介叾锛�Elastase锛夋績搴︺��
瑭﹀姂鐩掔祫鎴�锛�
瑭﹀姂鐩掔祫鎴�
48瀛旈厤缃�
96瀛旈厤缃�
淇濆瓨
瑾槑鏇�
1浠�
1浠�
灏佹澘鑶�
2鐗囷紙48锛�
2鐗囷紙96锛�
瀵嗗皝琚�
1鍊�(g猫)
1鍊�(g猫)
閰舵(bi膩o)鍖呰鏉�
1×48
1×96
2
妯�(bi膩o)婧�(zh菙n)鍝侊細54U/L
0.5ml×1鐡�
0.5ml×1鐡�
2
妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
1.5ml×1鐡�
1.5ml×1鐡�
2
閰舵(bi膩o)瑭﹀姂
3 ml×1鐡�
6 ml×1鐡�
2
妯ｅ搧绋�閲嬫恫
3 ml×1鐡�
6 ml×1鐡�
2
椤壊鍔�A娑�
3 ml×1鐡�
6 ml×1鐡�
2
椤壊鍔�B娑�
3 ml×1鐡�
6 ml×1鐡�
2
绲傛娑�
3ml×1鐡�
6ml×1鐡�
2
婵冪府娲楁粚娑�
锛�20ml×20鍊嶏級×1鐡�
锛�20ml×30鍊嶏級×1鐡�
2
妯ｆ湰铏曠悊鍙婅姹�锛�
1. 琛�娓咃細瀹ゆ韩琛�娑茶嚜鐒跺嚌鍥�10-20鍒嗛悩锛岄洟蹇�20鍒嗛悩宸﹀彸锛�2000-3000杞�(zhu菐n)/鍒嗭級銆備粩绱�(x矛)鏀堕泦涓婃竻锛屼繚瀛橀亷绋嬩腑濡傚嚭鐝�(xi脿n)娌夋穩锛屾噳(y墨ng)鍐嶆闆㈠績銆�
2. 琛�婕匡細鎳�(y墨ng)鏍规摎(j霉)妯�(bi膩o)鏈殑瑕佹眰閬告搰EDTA鎴栨妾吀閳変綔鐐烘姉鍑濆姂锛屾贩鍚�10-20鍒嗛悩鍚庯紝闆㈠績20鍒嗛悩宸﹀彸锛�2000-3000杞�(zhu菐n)/鍒嗭級銆備粩绱�(x矛)鏀堕泦涓婃竻锛屼繚瀛橀亷绋嬩腑濡傛湁娌夋穩褰㈡垚锛屾噳(y墨ng)瑭插啀娆￠洟蹇冦��
3. 灏挎恫锛氱敤鐒¤弻绠℃敹闆嗭紝闆㈠績20鍒嗛悩宸﹀彸锛�2000-3000杞�(zhu菐n)/鍒嗭級銆備粩绱�(x矛)鏀堕泦涓婃竻锛屼繚瀛橀亷绋嬩腑濡傛湁娌夋穩褰㈡垚锛屾噳(y墨ng)鍐嶆闆㈠績銆傝兏鑵规按銆佽叇鑴婃恫鍙冪収瀵�(sh铆)琛屻��
4. 绱�(x矛)鑳炲煿椁�(y菐ng)涓婃竻锛氭娓垎娉屾�х殑鎴愪唤鏅�(sh铆)锛岀敤鐒¤弻绠℃敹闆嗐�傞洟蹇�20鍒嗛悩宸﹀彸锛�2000-3000杞�(zhu菐n)/鍒嗭級銆備粩绱�(x矛)鏀堕泦涓婃竻銆傛娓窗(x矛)鑳炲収(n猫i)鐨勬垚浠芥檪(sh铆)锛岀敤PBS锛�PH7.2-7.4锛夌█閲嬬窗(x矛)鑳炴嚫娑诧紝绱�(x矛)鑳炴績搴﹂仈(d谩)鍒�100钀�/ml宸﹀彸銆傞�氶亷鍙嶅京(f霉)鍑嶈瀺锛屼互浣跨窗(x矛)鑳炵牬澹炲苟鏀惧嚭绱�(x矛)鑳炲収(n猫i)鎴愪唤銆傞洟蹇�20鍒嗛悩宸﹀彸锛�2000-3000杞�(zhu菐n)/鍒嗭級銆備粩绱�(x矛)鏀堕泦涓婃竻銆備繚瀛橀亷绋嬩腑濡傛湁娌夋穩褰㈡垚锛屾噳(y墨ng)鍐嶆闆㈠績銆�
5. 绲勭箶妯�(bi膩o)鏈細鍒囧壊妯�(bi膩o)鏈悗锛岀ū鍙栭噸閲忋�傚姞鍏ヤ竴瀹氶噺鐨�PBS锛�PH7.4銆傜敤娑叉爱杩呴�熷喎鍑嶄繚瀛�?zh猫n)婧嗚癌顥�?bi膩o)鏈瀺鍖栧悗浠嶇劧淇濇寔2
6. 妯�(bi膩o)鏈噰闆嗗悗鐩℃棭閫�(j矛n)琛屾彁鍙栵紝鎻愬彇鎸夌浉闂�(gu膩n)鏂囩嵒(xi脿n)閫�(j矛n)琛岋紝鎻愬彇鍚庢噳(y墨ng)鐩″揩閫�(j矛n)琛屽(sh铆)椹�(y脿n)銆傝嫢涓嶈兘棣笂閫�(j矛n)琛岃│椹�(y脿n)锛屽彲灏囨(bi膩o)鏈斁浜�
7. 涓嶈兘妾㈡脯鍚玁aN3鐨勬ǎ鍝�锛屽洜NaN3鎶戝埗杈ｆ牴閬庢哀鍖栫墿閰剁殑锛�HRP锛夋椿鎬с��
鎿嶄綔姝ラ锛�
1. 妯�(bi膩o)婧�(zh菙n)鍝佺殑绋�閲嬭垏鍔犳ǎ锛氬湪閰舵(bi膩o)鍖呰鏉夸笂瑷�(sh猫)妯�(bi膩o)婧�(zh菙n)鍝佸瓟10瀛旓紝鍦�*銆佺浜屽瓟涓垎鍒ュ姞妯�(bi膩o)婧�(zh菙n)鍝�100μl锛岀劧鍚庡湪*銆佺浜屽瓟涓姞妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫50μl锛屾贩鍕伙紱鐒跺悗寰�*瀛斻�佺浜屽瓟涓悇鍙�100μl鍒嗗垾鍔犲埌绗笁瀛斿拰绗洓瀛旓紝鍐嶅湪绗笁銆佺鍥涘瓟鍒嗗垾鍔犳(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫50μl锛屾贩鍕伙紱鐒跺悗鍦ㄧ涓夊瓟鍜岀鍥涘瓟涓厛鍚勫彇50μl妫勬帀锛屽啀鍚勫彇50μl鍒嗗垾鍔犲埌绗簲銆佺鍏瓟涓紝鍐嶅湪绗簲銆佺鍏瓟涓垎鍒ュ姞妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫50ul锛屾贩鍕伙紱娣峰嫽鍚庡緸绗簲銆佺鍏瓟涓悇鍙�50μl鍒嗗垾鍔犲埌绗竷銆佺鍏瓟涓紝鍐嶅湪绗竷銆佺鍏瓟涓垎鍒ュ姞妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫50μl锛屾贩鍕诲悗寰炵涓冦�佺鍏瓟涓垎鍒ュ彇50μl鍔犲埌绗節銆佺鍗佸瓟涓紝鍐嶅湪绗節绗崄瀛斿垎鍒ュ姞妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫50μl锛屾贩鍕诲悗寰炵涔濈鍗佸瓟涓悇鍙�50μl妫勬帀銆傦紙绋�閲嬪悗鍚勫瓟鍔犳ǎ閲忛兘鐐�50μl锛屾績搴﹀垎鍒ョ偤36 U/L,24 U/L ,12 U/L,6 U/L, 3 U/L锛夈��
2. 鍔犳ǎ锛氬垎鍒ヨō(sh猫)绌虹櫧瀛旓紙绌虹櫧灏嶇収瀛斾笉鍔犳ǎ鍝佸強閰舵(bi膩o)瑭﹀姂锛屽叾浣欏悇姝ユ搷浣滅浉鍚岋級銆�寰呮脯妯ｅ搧瀛斻�傚湪閰舵(bi膩o)鍖呰鏉夸笂寰呮脯妯ｅ搧瀛斾腑鍏堝姞妯ｅ搧绋�閲嬫恫40μl锛岀劧鍚庡啀鍔犲緟娓ǎ鍝�10μl锛堟ǎ鍝亃ui绲傜█閲嬪害鐐�5鍊嶏級銆�鍔犳ǎ灏囨ǎ鍝佸姞浜庨叾妯�(bi膩o)鏉垮瓟搴曢儴锛岀洝閲忎笉瑙稿強瀛斿锛岃紩杓曟檭鍕�(d貌ng)娣峰嫽銆�
3. 婧偛锛氱敤灏佹澘鑶滃皝鏉垮悗缃�
4. 閰嶆恫锛氬皣30锛�48T鐨�20鍊嶏級鍊嶆績绺礂婊屾恫鐢ㄨ捀椁炬按30锛�48T鐨�20鍊嶏級鍊嶇█閲嬪悗鍌欑敤銆�
5. 娲楁粚锛氬皬蹇冩彮鎺夊皝鏉胯啘锛屾鍘绘恫楂旓紝鐢╁共锛屾瘡瀛斿姞婊挎礂婊屾恫锛岄潨缃�30绉掑悗妫勫幓锛屽姝ら噸寰�(f霉)5娆★紝鎷嶅共銆�
6. 鍔犻叾锛氭瘡瀛斿姞鍏ラ叾妯�(bi膩o)瑭﹀姂50μl锛岀┖鐧藉瓟闄ゅ銆�
7. 婧偛锛氭搷浣滃悓3銆�
8. 娲楁粚锛氭搷浣滃悓5銆�
9. 椤壊锛氭瘡瀛斿厛鍔犲叆椤壊鍔�A50μl锛屽啀鍔犲叆椤壊鍔�B50μl锛岃紩杓曢渿钑╂贩鍕伙紝
10. 绲傛锛氭瘡瀛斿姞绲傛娑�50μl锛岀祩姝㈠弽鎳�(y墨ng)锛堟鏅�(sh铆)钘�(l谩n)鑹茬珛杞�(zhu菐n)榛冭壊锛夈��
11. 娓畾锛氫互绌虹櫧绌鸿(di脿o)闆讹紝450nm娉㈤暦渚濆簭娓噺鍚勫瓟鐨勫惛鍏夊害锛�OD鍊硷級銆� 娓畾鎳�(y墨ng)鍦ㄥ姞绲傛娑插悗15鍒嗛悩浠ュ収(n猫i)閫�(j矛n)琛屻��
娉ㄦ剰浜嬮爡(xi脿ng)锛�
1锛� 瑭﹀姂鐩掑緸鍐疯棌鐠�(hu谩n)澧冧腑鍙栧嚭鎳�(y墨ng)鍦ㄥ婧钩琛�15-30鍒嗛悩鍚庢柟鍙娇鐢紝閰舵(bi膩o)鍖呰鏉块枊灏佸悗濡傛湭鐢ㄥ畬锛屾澘姊濇噳(y墨ng)瑁濆叆瀵嗗皝琚嬩腑淇濆瓨銆�
2锛� 婵冩礂婊屾恫鍙兘鏈�(hu矛)鏈夌祼(ji茅)鏅舵瀽鍑猴紝绋�閲嬫檪(sh铆)鍙湪姘存荡涓姞婧姪婧讹紝娲楁粚鏅�(sh铆)涓嶅奖闊跨祼(ji茅)鏋溿��
3锛� 鍚勬鍔犳ǎ鍧囨噳(y墨ng)浣跨敤鍔犳ǎ鍣紝骞剁稉(j墨ng)甯告牎灏嶅叾婧�(zh菙n)纰烘�э紝浠ラ伩鍏嶈│椹�(y脿n)瑾ゅ樊銆備竴娆″姞妯ｆ檪(sh铆)闁撴帶鍒跺湪5鍒嗛悩鍏�(n猫i)锛屽妯�(bi膩o)鏈暩(sh霉)閲忓锛屾帹钖︿娇鐢ㄦ帓妲嶅姞妯ｃ��
4锛� 璜嬫瘡娆℃脯瀹氱殑鍚屾檪(sh铆)鍋氭(bi膩o)婧�(zh菙n)鏇茬窔锛屽仛寰�(f霉)瀛斻�傚妯�(bi膩o)鏈腑寰呮脯鐗╄唱(zh矛)鍚噺閬庨珮锛堟ǎ鏈�OD鍊煎ぇ浜庢(bi膩o)婧�(zh菙n)鍝佸瓟*瀛旂殑OD鍊硷級锛岃珛鍏堢敤妯ｅ搧绋�閲嬫恫绋�閲嬩竴瀹氬�嶆暩(sh霉)锛�n鍊嶏級鍚庡啀娓畾锛岃▓(j矛)绠楁檪(sh铆)璜媧ui鍚庝箻浠ョ附绋�閲嬪�嶆暩(sh霉)锛�×n×5锛夈��
5锛� 灏佹澘鑶滃彧闄愪竴娆℃�т娇鐢紝浠ラ伩鍏嶄氦鍙夋薄鏌撱��
6锛� 搴曠墿璜嬮伩鍏変繚瀛樸��
7锛� 鍤�(y谩n)鏍兼寜鐓ц鏄庢浉鐨勬搷浣滈��(j矛n)琛岋紝瑭﹂(y脿n)绲�(ji茅)鏋滃垽瀹氬繀闋堜互閰舵(bi膩o)鍎�璁�鏁�(sh霉)鐐烘簴(zh菙n).
8锛� 鎵�鏈夋ǎ鍝侊紝娲楁粚娑插拰鍚勭ó寤㈡鐗╅兘鎳�(y墨ng)鎸夊偝鏌撶墿铏曠悊銆�
9锛� 鏈│鍔戜笉鍚屾壒铏�(h脿o)绲勫垎涓嶅緱娣风敤銆�
10. 濡傝垏鑻辨枃瑾槑鏇告湁鐣帮紝浠ヨ嫳鏂囪鏄庢浉鐐烘簴(zh菙n)銆�
浠ユ(bi膩o)婧�(zh菙n)鐗╃殑婵冨害鐐烘┇鍧愭(bi膩o)锛�OD鍊肩偤绺卞潗妯�(bi膩o)锛�
鍦ㄥ潗妯�(bi膩o)绱欎笂绻嚭妯�(bi膩o)婧�(zh菙n)鏇茬窔锛屾牴鎿�(j霉)妯ｅ搧鐨�OD
鍊肩敱妯�(bi膩o)婧�(zh菙n)鏇茬窔鏌ュ嚭鐩告噳(y墨ng)鐨勬績搴︼紱鍐嶄箻浠ョ█閲�
鍊嶆暩(sh霉)锛涙垨鐢ㄦ(bi膩o)婧�(zh菙n)鐗╃殑婵冨害鑸�OD鍊艰▓(j矛)绠楀嚭妯�(bi膩o)
婧�(zh菙n)鏇茬窔鐨勭洿绶氬洖姝告柟绋嬪紡锛屽皣妯ｅ搧鐨�OD鍊�
浠ｅ叆鏂圭▼寮忥紝瑷�(j矛)绠楀嚭妯ｅ搧婵冨害锛屽啀涔樹互绋�閲�
鍊嶆暩(sh霉)锛屽嵆鐐烘ǎ鍝佺殑瀵�(sh铆)闅涙績搴︺��
锛堟鍦栧儏渚涘弮鑰冿級
瑭﹀姂鐩掓�ц兘锛�
1.妯ｅ搧绶氭�у洖姝歌垏闋�(y霉)鏈熸績搴︾浉闂�(gu膩n)绯绘暩(sh霉)R鍊肩偤0.95浠ヤ笂銆�
2.鎵瑰収(n猫i)鑸囨壒瑕嬫噳(y墨ng)鍒嗗垾灏忎簬9%鍜�11%
妾㈡脯鑼冨湇锛�
1 U/L -45 U/L
淇濆瓨姊濅欢鍙婃湁鏁堟湡锛�
1.瑭﹀姂鐩掍繚瀛橈細锛�2
2锛庢湁鏁堟湡锛�6鍊�(g猫)鏈�
2024涓栫挵(hu谩n)鏈�(hu矛)銆愬伐妤�(y猫)绡�(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆�
div6鏈�3鏃ワ紝2024涓栫挵(hu谩n)鏈�(hu矛)銆愬伐妤�(y猫)绡�(ji茅)鑳借垏鐠�(hu谩n)淇濆睍銆戜簬涓婃捣涓ㄥ湅瀹舵渻(hu矛)灞曚腑[瑭崇窗(x矛)]
涓婁竴绡囷細楂樺搧璩�(zh矛)澧婄墖鐭冲ⅷ澧婄墖鍗撶憺
涓嬩竴绡囷細鐭冲ⅷ澧婄墖鐨勫～鍏呮枡
- 鍑℃湰缍�(w菐ng)娉ㄦ槑"渚嗘簮锛氱挵(hu谩n)淇濆湪绶�"鐨勬墍鏈変綔鍝侊紝鐗堟瑠(qu谩n)鍧囧爆浜庣挵(hu谩n)淇濆湪绶氾紝杞�(zhu菐n)杓夎珛蹇呴爤娉ㄦ槑鐠�(hu谩n)淇濆湪绶氾紝http://www.hezebar.com銆傞仌鍙嶈�呮湰缍�(w菐ng)灏囪拷绌剁浉闂�(gu膩n)娉曞緥璨�(z茅)浠汇��
- 浼佹キ(y猫)鐧�(f膩)甯冪殑鍏徃鏂拌仦銆佹妧琛�(sh霉)鏂囩珷銆佽硣鏂欎笅杓夌瓑鍏�(n猫i)瀹癸紝濡傛秹鍙婁镜娆�(qu谩n)銆侀仌瑕�(gu墨)閬姇瑷寸殑锛屼竴寰嬬敱鐧�(f膩)甯冧紒妤�(y猫)鑷鎵挎摂(d膩n)璨�(z茅)浠伙紝鏈恫(w菐ng)鏈夋瑠(qu谩n)鍒櫎鍏�(n猫i)瀹瑰苟杩芥函璨�(z茅)浠汇��
- 鏈恫(w菐ng)杞�(zhu菐n)杓夊苟娉ㄦ槑鑷叾瀹冧締婧愮殑浣滃搧锛岀洰鐨勫湪浜庡偝閬炴洿澶氫俊鎭紝骞朵笉浠ｈ〃鏈恫(w菐ng)璐婂悓鍏惰榛�(di菐n)鎴栬瓑瀵�(sh铆)鍏跺収(n猫i)瀹圭殑鐪熷(sh铆)鎬э紝涓嶆壙鎿�(d膩n)姝ら浣滃搧渚垫瑠(qu谩n)琛岀偤鐨勭洿鎺ヨ铂(z茅)浠诲強閫ｅ付璨�(z茅)浠汇�傚叾浠栧獟楂斻�佺恫(w菐ng)绔欐垨鍊�(g猫)浜哄緸鏈恫(w菐ng)杞�(zhu菐n)杓夋檪(sh铆)锛屽繀闋堜繚鐣欐湰缍�(w菐ng)娉ㄦ槑鐨勪綔鍝佷締婧愶紝骞惰嚜璨�(f霉)鐗堟瑠(qu谩n)绛夋硶寰嬭铂(z茅)浠汇��
- 濡傛秹鍙婁綔鍝佸収(n猫i)瀹广�佺増娆�(qu谩n)绛夊晱椤岋紝璜�?ji膩n)璋ч珜閽抣(f膩)琛ㄤ箣鏃ヨ捣涓�鍛ㄥ収(n猫i)鑸囨湰缍�(w菐ng)鑱�(li谩n)绯伙紝鍚﹀墖瑕栫偤鏀炬鐩搁棞(gu膩n)娆�(qu谩n)鍒┿��
鐠�(hu谩n)淇濆湪绶欰PP
鐠�(hu谩n)淇濆湪绶氬皬绋嬪簭
寰俊鍏溇铏�(h脿o)
