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鑳冩硨绱犻噵鏀捐偨鍓嶉珨瀹氶噺瑭﹀姂鐩掕鏄庢浉
| 璩囨枡椤炲瀷 | doc鏂囦欢 | 璩囨枡澶у皬 | 86528 |
| 涓嬭級娆℃暩(sh霉) | 9 | 璩囨枡鍦栫墖 | 銆愰粸(di菐n)鎿婃煡鐪嬨�� |
| 涓� 鍌� 浜� | 涓婃捣鍗氭箹鐢熺墿绉戞妧鏈夐檺鍏徃 | 闇�瑕佺⿳鍒� | 0 |
| 闂�(gu膩n) 閸� 瑭� | 鑳冩硨绱犻噵鏀捐偨鍓嶉珨瀹氶噺瑭﹀姂鐩�,瑾槑鏇� | ||
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鑳冩硨绱犻噵鏀捐偨鍓嶉珨瀹氶噺瑭﹀姂鐩掕鏄庢浉
鏈│鍔戠洅鎳�(y墨ng)鐢ㄩ洐鎶楅珨澶惧績娉曟脯(c猫)瀹氭(bi膩o)鏈腑琛�娓呯礌/琛�娓呰兒(ST)姘村钩銆傜敤绱斿寲鐨�琛�娓呯礌/琛�娓呰兒(ST)鎶楅珨鍖呰寰瓟鏉�锛屽埗鎴愬浐鐩告姉楂旓紝寰�鍖呰鍠姉鐨勫井瀛斾腑渚濇鍔犲叆琛�娓呯礌/琛�娓呰兒(ST)锛�鍐嶈垏HRP妯�(bi膩o)瑷樼殑琛�娓呯礌/琛�娓呰兒(ST)鎶楅珨绲�(ji茅)鍚�锛屽舰鎴愭姉楂�-鎶楀師-閰舵(bi膩o)鎶楅珨寰�(f霉)鍚堢墿锛岀稉(j墨ng)閬�*娲楁粚鍚庡姞搴曠墿TMB椤壊銆俆MB鍦℉RP閰剁殑鍌寲涓嬭綁(zhu菐n)鍖栨垚钘�(l谩n)鑹诧紝骞跺湪閰哥殑浣滅敤涓嬭綁(zhu菐n)鍖栨垚鏈�绲傜殑榛冭壊銆傞鑹茬殑娣辨泛鍜屾ǎ鍝佷腑鐨�琛�娓呯礌/琛�娓呰兒(ST)鍛堟鐩搁棞(gu膩n)銆傜敤閰舵(bi膩o)鍎�鍦�450nm娉㈤暦(zh菐ng)涓嬫脯(c猫)瀹氬惛鍏夊害锛圤D鍊硷級锛岄�氶亷妯�(bi膩o)婧�(zh菙n)鏇茬窔瑷�(j矛)绠楁ǎ鍝佷腑琛�娓呯礌/琛�娓呰兒(ST)婵冨害銆�
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妯ｆ湰铏曠悊鍙婅姹傦細
1.琛�娓咃細鍏ㄨ妯�(bi膩o)鏈珛(q菒ng)浜庡婧斁缃�2灏忔檪(sh铆)鎴�4鈩冮亷澶滃悗浜�1000g闆㈠績20鍒嗛悩锛屽彇涓婃竻鍗冲彲妾㈡脯(c猫)锛屾垨?q奴)顣�?bi膩o)鏈斁浜�-20鈩冩垨-80鈩冧繚瀛橈紝浣嗘噳(y墨ng)閬垮厤鍙嶅京(f霉)鍑嶈瀺銆�
2. 琛�婕匡細鍙敤EDTA鎴栬倽绱犱綔鐐烘姉鍑濆姂锛屾(bi膩o)鏈噰闆嗗悗30鍒嗛悩鍏�(n猫i)浜�2 - 8掳C 1000g闆㈠績20鍒嗛悩锛屾垨?q奴)顣�?bi膩o)鏈斁浜�-20鈩冩垨-80鈩冧繚瀛�锛屼絾鎳�(y墨ng)閬垮厤鍙嶅京(f霉)鍑嶈瀺銆�
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4. 绱�(x矛)鑳炲煿椁�(y菐ng)鐗╀笂娓呮垨鍏跺畠鐢熺墿妯�(bi膩o)鏈細1000g闆㈠績20鍒嗛悩锛屽彇涓婃竻鍗冲彲妾㈡脯(c猫)锛屾垨?q奴)顣�?bi膩o)鏈斁浜�-20鈩冩垨-80鈩冧繚瀛橈紝浣嗘噳(y墨ng)閬垮厤鍙嶅京(f霉)鍑嶈瀺銆�瑭﹀姂鐩掓�ц兘锛�
1. 闈堟晱搴︼細zui灏忕殑妾㈡脯(c猫)婵冨害灏忎簬1铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝�銆傜█閲嬪害鐨勭窔鎬�銆傛ǎ鍝佺窔鎬у洖姝歌垏闋�(y霉)鏈熸績搴︾浉闂�(gu膩n)绯绘暩(sh霉)R鍊肩偤0.990銆�
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1. 妯�(bi膩o)婧�(zh菙n)鍝佺殑绋�閲嬶細鏈│鍔戠洅鎻愪緵鍘熷�嶆(bi膩o)婧�(zh菙n)鍝佷竴鏀�锛岀敤鎴跺彲鎸夌収涓嬪垪鍦栬〃鍦ㄥ皬瑭︾涓��(j矛n)琛岀█閲�銆�
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40pg/ml
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150渭l鐨�5铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�150渭l妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
20pg/ml
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150渭l鐨�4铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�150渭l妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
10pg/ml
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150渭l鐨�3铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�150渭l妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
5pg/ml
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150渭l鐨�2铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�150渭l妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
2. 鍔犳ǎ锛氬垎鍒ヨō(sh猫)绌虹櫧瀛旓紙绌虹櫧灏�(du矛)鐓у瓟涓嶅姞妯ｅ搧鍙婇叾妯�(bi膩o)瑭﹀姂锛屽叾浣欏悇姝ユ搷浣滅浉鍚岋級銆佹(bi膩o)婧�(zh菙n)瀛斻�佸緟娓�(c猫)妯ｅ搧瀛斻�傚湪閰舵(bi膩o)鍖呰鏉夸笂妯�(bi膩o)婧�(zh菙n)鍝佹簴(zh菙n)纰哄姞妯�50渭l锛屽緟娓�(c猫)妯ｅ搧瀛斾腑鍏堝姞妯ｅ搧绋�閲嬫恫40渭l锛岀劧鍚庡啀鍔犲緟娓�(c猫)妯ｅ搧10渭l锛堟ǎ鍝佹渶绲傜█閲嬪害鐐�5鍊嶏級銆傚姞妯ｅ皣妯ｅ搧鍔犱簬閰舵(bi膩o)鏉垮瓟搴曢儴锛岀洝閲忎笉瑙稿強瀛斿锛岃紩杓曟檭鍕�(d貌ng)娣峰嫽銆�
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5. 娲楁粚锛氬皬蹇冩彮鎺夊皝鏉胯啘锛屾鍘绘恫楂旓紝鐢╁共锛屾瘡瀛斿姞婊挎礂婊屾恫锛岄潨缃�30绉掑悗妫勫幓锛屽姝ら噸寰�(f霉)5娆★紝鎷嶅共銆�
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